mult1  (R&D Systems)

Journal: Journal of Inflammation Research

Article Title: MULT1-Encoding DNA Alleviates Schistosomiasis-Associated Hepatic Fibrosis via Modulating Cellular Immune Response

doi: 10.2147/JIR.S354224

Figure Lengend Snippet: MULT1 encoding DNA injection alleviates egg granuloma and hepatic fibrosis in mice infected with Schistosoma japonicum . ( A ) Experimental design. Briefly, 6-week-old BALB/c female mice (n=6 per group) were artificially infected with S. japonicum by cutaneous contact with 24 cercariae on a wet cover slip. Administration of 40 μg rMULT1 DNA or vehicle DNA was carried out via hydrodynamic tail vein injection; the process was initialized at 4 weeks post infection and repeated 3 times in 1 month before the mice were anaesthetized and sacrificed at the end point. ( B ) RT-qPCR data and ( C ) sandwich fluorescence immunoassay showing elevated MULT1 expression in p-rMULT1-injected mice compared with control group mice (GFP-ctl) administered vehicle plasmids. ( D ) Representative H&E staining images (left panel, magnification x200) and quantification of mean (±SEM) egg-induced granuloma size in the liver (white circles). ( E ) Representative Masson’s trichrome staining images (left panel, magnification x200) and quantification of mean (±SEM) collagen deposition (right panel). ( F and G ) RT-qPCR showing decrease of ( F ) liver collagen I and ( G ) α-SMA expression. Western blotting assay demonstrating reduced protein concentration of ( H and I ) collagen I, ( H and J ) α-SMA and ( H and K ) TGF-β in the livers of mice administered rMULT1 DNA. Data are representative of 4–6 animals per subgroup and 3 independent experiments. Comparisons were between rMULT1 and GFP-ctl, *P<0.05 and **P<0.01.

Article Snippet: A standard curve was drawn from the MFI of the standard recombinant MULT1 protein (R&D Systems, Inc.) and the concentration of MULT1 was calculated based on the standard curve.

Techniques: Injection, Infection, Quantitative RT-PCR, Fluorescence, Expressing, Staining, Western Blot, Protein Concentration

Journal: Journal of Inflammation Research

Article Title: MULT1-Encoding DNA Alleviates Schistosomiasis-Associated Hepatic Fibrosis via Modulating Cellular Immune Response

doi: 10.2147/JIR.S354224

Figure Lengend Snippet: MULT1 encoding DNA injection alleviates egg granuloma and hepatic fibrosis in mice infected with Schistosoma japonicum . ( A ) Experimental design. Briefly, 6-week-old BALB/c female mice (n=6 per group) were artificially infected with S. japonicum by cutaneous contact with 24 cercariae on a wet cover slip. Administration of 40 μg rMULT1 DNA or vehicle DNA was carried out via hydrodynamic tail vein injection; the process was initialized at 4 weeks post infection and repeated 3 times in 1 month before the mice were anaesthetized and sacrificed at the end point. ( B ) RT-qPCR data and ( C ) sandwich fluorescence immunoassay showing elevated MULT1 expression in p-rMULT1-injected mice compared with control group mice (GFP-ctl) administered vehicle plasmids. ( D ) Representative H&E staining images (left panel, magnification x200) and quantification of mean (±SEM) egg-induced granuloma size in the liver (white circles). ( E ) Representative Masson’s trichrome staining images (left panel, magnification x200) and quantification of mean (±SEM) collagen deposition (right panel). ( F and G ) RT-qPCR showing decrease of ( F ) liver collagen I and ( G ) α-SMA expression. Western blotting assay demonstrating reduced protein concentration of ( H and I ) collagen I, ( H and J ) α-SMA and ( H and K ) TGF-β in the livers of mice administered rMULT1 DNA. Data are representative of 4–6 animals per subgroup and 3 independent experiments. Comparisons were between rMULT1 and GFP-ctl, *P<0.05 and **P<0.01.

Article Snippet: Briefly, nonionic latex beads (Invitrogen; Thermo Fisher Scientific, Inc.) were coated with 25 μg/mL MULT1 monoclonal antibody (clone 5D10; eBioscience; Thermo Fisher Scientific, Inc.) and blocked with 2% bovine serum album and then incubated with liver tissue lysates and then captured MULT1 was detected by staining with fluorescence labeled detecting antibody specific for MULT1 (clone 237104, R&D Systems, Inc.) and analyzing on FACSVerse or LSRII flow cytometer.

Techniques: Injection, Infection, Quantitative RT-PCR, Fluorescence, Expressing, Staining, Western Blot, Protein Concentration

Journal: Scientific Reports

Article Title: Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer

doi: 10.1038/s41598-020-76906-5

Figure Lengend Snippet: Immunohistochemical staining and expression of NKG2D-receptor and ligands in adipose tissue. Relative expression and representative pictures of immunohistochemically staining of NKG2D-receptor ( a and b ); MULT1 ( c and d ) and Rae1 ( e and f ) in visceral adipose tissues of mice in the long-term experiment receiving either control diet (Co) or DIO diet (DIO) and injection of sodium chloride (NaCl) or 4T1-luc2 breast cancer cells (Tumor). Values represent means ± SEM, n = 5 mice/group and five analysed tissue sections per mice. * indicates significant differences of means between mice receiving DIO diet (DIO) compared to mice receiving control diet (Co) analyzed by two-way ANOVA (**** p ≤ 0.0001). Different superscript letters ( a , b ) indicate significant differences between individual experimental groups analyzed by Tukey´s multiple comparison test (p ≤ 0.05). Scale bar (40 µm).

Article Snippet: Paraffin sections (5 μm) of visceral adipose tissue were prepared and stained with the mAbs anti-NKG2D (ab203353, Abcam, Cambridge, United Kingdom) and anti-UL16 Binding Protein 1 (ULBP1/MULT1, ABIN966609, antibodies online, Aachen, Germany) using the EnVision + System (horseradish peroxidase (HRP) labelled polymer anti-rabbit, Dako North America Inc., Carpinteria, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Injection